FMR ELISA Kits

FMRP ELISA kits are designed to detect and quantify FMRP concentrations in various biological samples, including cell lysates, tissue homogenates, serum, and plasma. These kits typically employ a sandwich ELISA format, where specific antibodies capture FMRP, and detection is facilitated through enzyme-linked secondary antibodies, resulting in a measurable signal proportional to the FMRP concentration.

Key Components:

• Pre-coated Microplate: Wells pre-coated with anti-FMRP antibodies to capture the target protein.
• Standards and Controls: Known concentrations of FMRP to generate a standard curve and validate assay performance.
• Detection Antibodies: Enzyme-conjugated antibodies specific to FMRP for signal generation.
• Substrate Solution: Reacts with the enzyme to produce a detectable signal, typically colorimetric.

Applications:

• Neuroscience Research: Investigating the role of FMRP in synaptic function, neural development, and its involvement in neurodevelopmental disorders.
• Clinical Diagnostics: Assessing FMRP levels to aid in the diagnosis of Fragile X Syndrome and related conditions.
• Pharmaceutical Development: Evaluating the impact of therapeutic agents on FMRP expression and function.

Examples of FMRP ELISA Kits:

• Human FMR1 / FMRP Quant ELISA Kit - LSBio: A 96-well sandwich ELISA designed for the quantitative detection of human FMR1/FMRP in cell lysates and tissue homogenates, with a sensitivity of 0.117 ng/mL.
• Human FMRP ELISA Kit - Abcam: A single-wash 90-minute sandwich ELISA designed for the quantitative measurement of FMRP protein in cell lysate and cell culture extracts, with a sensitivity of 99.67 pg/mL.
• Human FMR1 ELISA Kit - Biomatik: A sandwich ELISA for detecting Fragile X Mental Retardation 1 in serum, plasma, and tissue homogenates, with a detection range of 15.6–1000 pg/mL and a sensitivity of 3.9 pg/mL.

By providing reliable and quantitative measurements of FMRP, ELISA kits are indispensable tools in advancing our understanding of its biological functions and facilitating research in various biomedical fields.