The AffiELISA® Mouse HAVCR2 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Hepatitis A Virus Cellular Receptor 2 (HAVCR2) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Hepatitis A Virus Cellular Receptor 2 (HAVCR2) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Hepatitis A Virus Cellular Receptor 2 (HAVCR2) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Hepatitis A Virus Cellular Receptor 2 (HAVCR2) in the samples can be determined.
AffiELISA® Mouse HAVCR2 Kit
Hepatitis A Virus Cellular Receptor 2
KIM-3 - TIM3 - TIMD3 - Tim-3 - T-cell membrane protein 3 - T-cell immunoglobulin mucin receptor 3 - T-cell immunoglobulin and mucin domain-containing protein 3
Mouse
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids
Infection immunity.