The AffiELISA® Human KDM2B Lysine Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Lysine (K) specific demethylase 2B (KDM2B) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Lysine (K) specific demethylase 2B (KDM2B) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Lysine (K) specific demethylase 2B (KDM2B) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Lysine (K) specific demethylase 2B (KDM2B) in the samples can be determined.
AffiELISA® Human KDM2B Lysine Kit
KDM2B Lysine
[Histone-H3]-lysine-36 demethylase 1B - CXXC-type zinc finger protein 2 - CXXC2 - F box and leucine rich repeat protein 10 - F box protein FBL10 - F-box and leucine-rich repeat protein 10 - F-box protein FBL10 - F-box/LRR-repeat protein 10 - Fbl10 - FBXL10 - JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein - JHDM1B - JmjC domain-containing histone demethylation protein 1B - Jumonji C domain containing histone demethylase 1B - Jumonji domain-containing EMSY-interactor methyltransferase motif protein - kdm2b - KDM2B_HUMAN - Lysine (K) specific demethylase 2B - Lysine-specific demethylase 2B - PCCX2 - Protein containing CXXC domain 2 - Protein JEMMA - Protein-containing CXXC domain 2 -
Human
Sandwich ELISA
Serum, plasma and other biological fluids
Epigenetics and Nuclear Signaling.