KDM ELISA Kits

KDM (Lysine Demethylase) ELISA kits are specialized immunoassays designed for the quantitative detection of KDM enzyme levels in biological samples such as tissue lysates, serum, or cell culture supernatants. KDMs are involved in the removal of methyl groups from lysine residues on histones and other proteins, playing critical roles in gene regulation, epigenetic modifications, and cellular differentiation. These kits enable researchers to quantify KDM activity or expression to study their impact on various biological processes and diseases, particularly those involving epigenetic regulation and cancer.

Content of KDM ELISA Kits: KDM ELISA kits generally include:

• 96-well microplates pre-coated with an anti-KDM capture antibody.
• Recombinant KDM standards for calibration curve creation.
• A detection antibody specific to KDM, often conjugated to an enzyme like HRP (Horseradish Peroxidase).
• Substrate solution such as TMB (3,3',5,5'-Tetramethylbenzidine) for colorimetric detection.
• Stop solution to terminate the reaction.
• Sample diluent, assay buffers, and wash buffer.
• Comprehensive instruction manual with protocols for sample handling and assay execution.

Application of KDM ELISA Kits: KDM ELISA kits have several important research and diagnostic applications:

• Epigenetics research: Quantifying KDMs to understand their role in chromatin remodeling, gene silencing, and transcriptional regulation.
• Cancer research: Studying KDM expression and activity in tumor cells, as dysregulated KDMs are often associated with oncogenesis, metastasis, and cancer progression.
• Neurodegenerative diseases: Investigating KDMs in the context of diseases such as Alzheimer's and Parkinson’s, where epigenetic changes contribute to disease pathology.
• Stem cell and developmental biology: Analyzing KDMs' involvement in stem cell differentiation and development by examining their regulatory influence on gene expression.
• Drug discovery: Screening potential inhibitors or modulators of KDM activity for therapeutic applications in diseases linked to epigenetic dysregulation.

KDM ELISA kits are essential tools for researchers focused on understanding the complex roles of lysine demethylases in epigenetics, providing accurate and reproducible quantitative data that support the elucidation of molecular mechanisms in health and disease.