AffiELISA® KDM ELISA Kits

KDM ELISA (Enzyme-Linked Immunosorbent Assay) kits are designed to detect and quantify lysine demethylases (KDMs), a family of enzymes involved in the removal of methyl groups from lysine residues on histones and other proteins. KDMs play crucial roles in regulating gene expression, chromatin structure, and various cellular processes. Accurate measurement of KDMs is important for studying epigenetic regulation, cancer research, and other areas of molecular biology.

Components of KDM ELISA Kits

Microplate: A 96-well plate pre-coated with antibodies specific to the target KDM. This allows for the specific capture of KDMs from the sample.
Reagents:
- Capture Antibodies: These antibodies are coated onto the microplate wells and specifically bind to the target KDM.
- Detection Antibodies: These antibodies are conjugated to an enzyme (e.g., horseradish peroxidase, HRP) and bind to the captured KDM for detection.
- Substrate Solution: Reacts with the enzyme linked to the detection antibodies to produce a colorimetric change that correlates with the amount of KDM.
- Stop Solution: An acid solution that halts the enzymatic reaction and stabilizes the color change for measurement.

Sensitivity and Specificity

Sensitivity: Indicates the kit’s ability to detect low levels of KDM. High sensitivity is crucial for detecting subtle changes in KDM levels.
Specificity: Ensures that the assay specifically detects the target KDM, minimizing cross-reactivity with other proteins or isoforms

Applications

Epigenetic Research: Measures KDM levels to study their role in histone modification and chromatin dynamics.
Cancer Research: Investigates the role of KDMs in cancer development and progression, as alterations in KDM activity can impact gene expression and tumorigenesis.
Drug Development: Evaluates the effects of potential drugs targeting KDMs, helping to assess their efficacy and safety.

KDM ELISA kits are essential for the quantification of lysine demethylases in various research and clinical applications. They are widely used in epigenetics, cancer research, and drug development. Understanding the kit’s components, procedures, and limitations ensures accurate and reliable measurement of KDMs, providing valuable insights into their biological functions and roles in disease processes.