The AffiELISA® Human TNKS1 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Tankyrase 1 (TNKS1) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Tankyrase 1 (TNKS1) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Tankyrase 1 (TNKS1) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Tankyrase 1 (TNKS1) in the samples can be determined.
AffiELISA® Human TNKS1 Kit
Tankyrase 1
TNKS - ARTD5 - PARPL - PARP-5a - PARP5A - TIN1 - TINF1 - TNKS-1 - ADP-ribosyltransferase diphtheria toxin-like 5 - TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase
Human
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids.
Enzyme and Kinase.