TNKS ELISA Kits
Tankyrase (TNKS) enzymes, comprising TNKS1 and TNKS2, are poly(ADP-ribose) polymerases involved in various cellular processes, including telomere maintenance, Wnt signaling, and regulation of glucose metabolism. Quantifying TNKS levels is crucial in research areas such as oncology, aging, and metabolic disorders. TNKS ELISA kits are specialized immunoassays designed for the sensitive and specific detection of TNKS proteins in various biological samples.
Technical Overview:
TNKS ELISA kits typically employ a sandwich enzyme-linked immunosorbent assay format:
- Capture Antibody: A monoclonal or polyclonal antibody specific to TNKS is pre-coated onto a microplate.
- Sample Addition: Biological samples (e.g., serum, plasma, tissue homogenates) are added to the wells, allowing any TNKS present to bind to the capture antibodies.
- Detection Antibody: A biotinylated detection antibody, also specific to TNKS, is introduced, forming a sandwich complex.
- Enzyme Conjugate: Streptavidin-Horseradish Peroxidase (HRP) conjugate binds to the biotinylated detection antibody.
- Substrate Addition: A chromogenic substrate (e.g., TMB) is added, and the enzyme catalyzes a colorimetric reaction.
- Quantification: The intensity of the color change is measured spectrophotometrically, correlating with the concentration of TNKS in the sample.
Applications:
- Cancer Research: Evaluating TNKS expression in tumor samples to understand its role in cancer progression and as a potential therapeutic target.
- Aging Studies: Investigating the involvement of TNKS in telomere maintenance and cellular senescence.
- Metabolic Research: Studying TNKS's role in glucose metabolism and its implications in metabolic disorders.
These kits provide researchers with reliable and specific tools to quantify TNKS levels, facilitating advancements in understanding their roles in various biological processes and diseases.
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