AffiELISA Kits

AffiELISA® Kits represent a comprehensive range of enzyme-linked immunosorbent assays (ELISAs) designed to quantify specific proteins, antibodies, or antigens within biological samples. Engineered with high specificity and sensitivity, AffiELISA® Kits utilize monoclonal or polyclonal antibodies tailored for precise binding, ensuring accuracy and consistency across various biological matrices, including serum, plasma, tissue lysates, and cell culture supernatants.

Content of AffiELISA® Kits

Each AffiELISA® Kit comprises several key components designed for streamlined, high-throughput analysis:

• Coated Microplate: 96-well or 384-well plates pre-coated with specific antibodies or antigens, providing robust and reproducible signal generation.
• Detection Reagents: Optimized detection antibodies conjugated with enzyme markers, often horseradish peroxidase (HRP) or alkaline phosphatase (AP), to amplify target detection.
• Standard and Control Samples: Quantified standards and controls for accurate calibration and reliable result validation.
• Substrate Solution: Ready-to-use substrates, typically TMB (3,3′,5,5′-tetramethylbenzidine), for colorimetric signal development, enabling quantifiable optical density measurements.
• Assay Buffer: Specialized buffers to maintain sample integrity, enhance specificity, and minimize cross-reactivity.

Application of AffiELISA® Kits

AffiELISA® Kits are widely used across multiple research domains for:

• Biomarker Detection: Quantifying biomarkers in clinical research, enabling early diagnosis and disease progression analysis.
• Pathogen Detection: Detecting viral or bacterial antigens in infectious disease research.
• Pharmacokinetics: Measuring drug levels or immune responses in pharmacological studies, including monitoring therapeutic antibody levels.
• Immunology Studies: Profiling cytokine release or immune responses in cell-based experiments, critical in immunotherapy and vaccine development.

These kits provide reproducible, quantifiable results ideal for translational research, diagnostic development, and high-throughput screening applications.