96 Well Plate

A 96-well plate is a microplate with a grid of 96 individual wells, arranged in an 8x12 configuration. Each well acts as a mini-test tube, holding a small volume of liquid sample, typically between 50-300 µL. This standardized format is extensively used in molecular biology, immunology, drug discovery, and clinical diagnostics for high-throughput experiments, as it allows for simultaneous analysis of multiple samples or replicates in a compact and organized manner.

Structure and Material of 96-Well Plates
  • Materials: Commonly manufactured from clear polystyrene for optical assays, or opaque white or black polystyrene for luminescence and fluorescence assays, respectively. Material composition is chosen based on assay type to minimize background interference and enhance signal detection.
  • Coating: Plates can be uncoated or pre-coated with biomolecules (such as antibodies or antigens), poly-L-lysine, or other substances to promote cell attachment, protein binding, or specific molecule detection.
  • Well Types:
    • Flat-Bottom Wells: Ideal for optical assays, such as ELISA, where accurate measurement by spectrophotometry is required.
    • Round-Bottom Wells: Used for assays where mixing is essential, as the round shape improves mixing efficiency.
    • U-Bottom and V-Bottom Wells: V-shaped wells are especially useful for pellet formation in centrifugation, commonly used in cell and particle applications.
Applications of 96-Well Plates

96-well plates are versatile tools used in:

  • Enzyme-Linked Immunosorbent Assay (ELISA): For detecting and quantifying proteins, antibodies, or other analytes in biological samples.
  • Cell Culture: Some plates are optimized for cell adhesion, making them suitable for high-throughput cell proliferation, toxicity, or viability studies.
  • PCR and qPCR: For nucleic acid amplification, particularly when testing multiple primers, probes, or samples.
  • Drug Screening: For assessing the effects of various compounds across a range of concentrations on target cells or biomolecules.
  • Automated Liquid Handling: Compatible with robotic systems for high-throughput screening, where consistent sample processing is critical.
603.40 603.4 USD
603.40 603.4 USD
603.40 603.4 USD
603.40 603.4 USD
603.40 603.4 USD
603.40 603.4 USD
603.40 603.4 USD
603.40 603.4 USD
603.40 603.4 USD
625.45 625.45 USD
625.45 625.45 USD
AffiELISA® Peanut Ara h2 Detection Kit
CAT# AFG-CND-211
Size: 1 kit
625.45 625.45 USD
AffiELISA® Peant Ara h6 Detection Kit
CAT# AFG-CND-210
Size: 1 kit
625.45 625.45 USD
395.50 395.5 USD
AffiELISA® Ovalbumin (OVA) Detection Kit
CAT# AFG-CND-200
Size: 1 kit
625.45 625.45 USD
734.65 734.65 USD
654.85 654.85 USD
654.85 654.85 USD
654.85 654.85 USD
654.85 654.85 USD
654.85 654.85 USD
654.85 654.85 USD
654.85 654.85 USD
654.85 654.85 USD
654.85 654.85 USD
654.85 654.85 USD
637.00 637.0 USD
637.00 637.0 USD
580.30 580.3000000000001 USD
580.30 580.3000000000001 USD
666.40 666.4 USD
666.40 666.4 USD
AffiELISA® Mouse Albumin Detection Kit
CAT# AFG-CND-103
Size: 1 kit
561.40 561.4 USD
666.40 666.4 USD
671.65 671.65 USD
671.65 671.65 USD
536.00 536.0 USD
AffiELISA® Porcine P-Selectin | P-Sel | ELISA Kit
CAT# AFG-SM-14759
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine PSA ELISA Kit
CAT# AFG-SM-14758
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Perforin ELISA Kit
CAT# AFG-SM-14659
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Peptidoglycan ELISA Kit
CAT# AFG-SM-14657
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Peptide γ ELISA Kit
CAT# AFG-SM-14656
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Peptide YY ELISA Kit
CAT# AFG-SM-14655
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Pepsinogen C | PGC | ELISA Kit
CAT# AFG-SM-14654
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Pepsinogen A | PGA | ELISA Kit
CAT# AFG-SM-14653
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Pepsin ELISA Kit
CAT# AFG-SM-14652
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Pentosidine | PTD | ELISA Kit
CAT# AFG-SM-14651
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine P-cadherin | P-cad | ELISA Kit
CAT# AFG-SM-14650
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Paraoxonase | PON | ELISA Kit
CAT# AFG-SM-14649
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Papillomavirus | HPV | ELISA Kit
CAT# AFG-SM-14648
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Pan-cytokeIn | P-CK | ELISA Kit
CAT# AFG-SM-14647
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Pancreastatin ELISA Kit
CAT# AFG-SM-14643
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Oxytocin | OT | ELISA Kit
CAT# AFG-SM-14641
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Osteopontin | OPN | ELISA Kit
CAT# AFG-SM-14635
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Osteonectin | ON | ELISA Kit
CAT# AFG-SM-14634
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Osteocalcin | OT | ELISA Kit
CAT# AFG-SM-14631
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine OSM ELISA Kit
CAT# AFG-SM-14630
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Orexin Receptor | OR | ELISA Kit
CAT# AFG-SM-14627
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Orexin B ELISA Kit
CAT# AFG-SM-14626
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Orexin A ELISA Kit
CAT# AFG-SM-14625
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Omentin ELISA Kit
CAT# AFG-SM-14623
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Oligomeric protein ELISA Kit
CAT# AFG-SM-14622
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Norepinephrine | NE | ELISA Kit
CAT# AFG-SM-14615
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Noradrenaline | NE | ELISA Kit
CAT# AFG-SM-14614
Size: 96 Wells
536.00 536.0 USD