ELISA 48 & 96 Wells

The 48-well and 96-well ELISA formats are specialized microplate configurations commonly used in enzyme-linked immunosorbent assays (ELISAs) for high-sensitivity detection of specific proteins, antibodies, or antigens. The choice between a 48-well and a 96-well plate format depends on sample volume, assay scale, and throughput requirements.

Structural Differences
  1. Well Count and Arrangement:
    • 48-Well Plates: Configured in a 6x8 grid, allowing for a medium-throughput format suited for experiments where fewer samples or replicates are tested, with a higher volume per well.
    • 96-Well Plates: Arranged in an 8x12 grid, widely adopted for high-throughput screening, enabling simultaneous processing of a larger number of samples or replicates with smaller sample volumes.
  2. Well Volume:
    • 48-Well: Holds larger volumes per well, typically between 500-1000 µL, providing flexibility for assays requiring higher sample or reagent volumes.
    • 96-Well: Accommodates 50-300 µL per well, optimized for standard ELISA protocols that benefit from minimal reagent usage and higher efficiency.
Applications of 48-Well vs. 96-Well ELISA Plates
  • 48-Well ELISA Plates:
    • Preferred in pilot studies or preliminary screenings where sample quantity is limited or fewer test conditions are required.
    • Ideal for applications needing higher sample volumes for increased sensitivity or lower detection limits.
    • Suitable for assays where less automation is used and smaller sample sets are being analyzed.
  • 96-Well ELISA Plates:
    • Widely used in large-scale studies and high-throughput screening, where analyzing a greater number of samples or replicates simultaneously is essential.
    • Commonly implemented in biomarker discovery, immunoassays, and pharmacokinetics, where processing efficiency and reproducibility are critical.
    • Compatible with automated liquid handling systems, enabling high-throughput data collection and reducing manual error.
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637.00 637.0 USD
AffiELISA® SEB Detection Kit
CAT# AFG-CND-198
Size: 1 kit
625.45 625.45 USD
734.65 734.65 USD
734.65 734.65 USD
734.65 734.65 USD
AffiELISA® Rat Type I Collagen Detection Kit
CAT# AFG-CND-183
Size: 1 kit
734.65 734.65 USD
AffiELISA® Trypsin Activity ELISA Kit
CAT# AFG-CND-133
Size: 1 kit
580.30 580.3000000000001 USD
AffiELISA® Rat Albumin Detection Kit
CAT# AFG-CND-111
Size: 1 kit
580.30 580.3000000000001 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Ratin antibody | AKA | ELISA Kit
CAT# AFG-SM-15062
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Ratin 18 | CK-18 | ELISA Kit
CAT# AFG-SM-15061
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Rathyroid Hormone | PTH | ELISA Kit
CAT# AFG-SM-15060
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Rathormone | i-PTH | ELISA Kit
CAT# AFG-SM-15059
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Rate | 2, 3-DPG | ELISA Kit
CAT# AFG-SM-15057
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine β Naphthol ELISA Kit
CAT# AFG-SM-15050
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine β Glucosidase ELISA Kit
CAT# AFG-SM-15041
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine α-Fetoprotein | AFP | ELISA Kit
CAT# AFG-SM-15033
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Zonulin ELISA Kit
CAT# AFG-SM-15021
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Zidovudine | AZT | AZT ELISA Kit
CAT# AFG-SM-15020
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Vitronectin | VTN | ELISA Kit
CAT# AFG-SM-15017
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Visfatin | VF | ELISA Kit
CAT# AFG-SM-15006
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Vimentin | VIM | ELISA Kit
CAT# AFG-SM-15004
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine urotensin II | UII | ELISA Kit
CAT# AFG-SM-14993
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Urokinase ELISA Kit
CAT# AFG-SM-14991
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Urinary PGl2 | uPGI2 | ELISA Kit
CAT# AFG-SM-14989
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Urea ELISA Kit
CAT# AFG-SM-14985
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine TSI ELISA Kit
CAT# AFG-SM-14964
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine Trypsin | TPS | ELISA Kit
CAT# AFG-SM-14962
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
AffiELISA® Porcine True Insulin | TI | ELISA Kit
CAT# AFG-SM-14960
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine Troponin T | Tn-T | ELISA Kit
CAT# AFG-SM-14959
Size: 96 Wells
536.00 536.0 USD
AffiELISA® Porcine troponin I | Tn-I | ELISA Kit
CAT# AFG-SM-14958
Size: 96 Wells
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD
536.00 536.0 USD