High Spesific ELISA 

High Specificity ELISA is a refined version of the standard ELISA (Enzyme-Linked Immunosorbent Assay) that focuses on achieving precise and accurate detection of a specific target analyte amidst a complex mixture. This enhanced specificity is crucial for applications where distinguishing the target from similar molecules is critical.

Key Features of High Specificity ELISA
  • Highly Specific Antibodies:
    • Objective: Use antibodies that bind only to the target analyte with minimal cross-reactivity.
    • Mechanism: Employ monoclonal antibodies or highly purified polyclonal antibodies with high selectivity for the target.
  • Optimized Coating:
    • Capture Antibodies or Antigens: Use well-characterized capture antibodies or antigens that are specifically tailored to the target analyte.
    • Proper Immobilization: Ensure effective and stable immobilization of the capture molecules on the assay plate.
  • Stringent Washing Steps:
    • Objective: Minimize non-specific binding and reduce background noise.
    • Mechanism: Perform thorough washing with optimized buffer solutions to remove unbound substances while retaining specific interactions.
  • Advanced Blocking Techniques:
    • Blocking Agents: Use specialized blocking agents or buffers to prevent non-specific binding.
    • Condition Optimization: Optimize the blocking conditions (time, concentration, and composition) to reduce background interference.
  • Selective Detection Reagents:
    • Secondary Antibodies: Utilize secondary antibodies that are specifically conjugated to enzymes or labels with high affinity for the primary antibody or antigen.
    • Minimal Cross-Reactivity: Ensure that detection reagents are specifically tailored to recognize only the primary antibody or antigen.
Applications
  • Diagnostic Testing: Detect specific biomarkers or pathogens in clinical samples with high accuracy, such as detecting specific viral proteins or hormones.
  • Research: Study specific proteins, peptides, or other biomolecules in complex biological samples.
  • Quality Control: Verify the presence of specific components in pharmaceuticals, food products, or other industrial applications.
AffiELISA®​ Rat PNPLA2 ELISA Kit
CAT# AFG-IVE-14235
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat PMP22 ELISA Kit
CAT# AFG-IVE-14232
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat PFKM ELISA Kit
CAT# AFG-IVE-14179
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat PFKL ELISA Kit
CAT# AFG-IVE-14178
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat PDGFD ELISA Kit
CAT# AFG-IVE-14156
Size: 96 Wells
1,564.75 1564.75 USD
1,601.88 1601.88 USD
1,601.88 1601.88 USD
AffiELISA®​ Rat PDGFB ELISA Kit
CAT# AFG-IVE-14151
Size: 96 Wells
1,564.75 1564.75 USD
1,601.88 1601.88 USD
AffiELISA®​ Rat PDE4B ELISA Kit
CAT# AFG-IVE-14145
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat PAX6 ELISA Kit
CAT# AFG-IVE-14130
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat P4HA1 ELISA Kit
CAT# AFG-IVE-14120
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat OSTN ELISA Kit
CAT# AFG-IVE-14114
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NUCB2 ELISA Kit
CAT# AFG-IVE-14082
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat NRP2 ELISA Kit
CAT# AFG-IVE-14070
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NRG2 ELISA Kit
CAT# AFG-IVE-14066
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NR4A1 ELISA Kit
CAT# AFG-IVE-14061
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NR1I3 ELISA Kit
CAT# AFG-IVE-14058
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NR1D2 ELISA Kit
CAT# AFG-IVE-14053
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NPY5R ELISA Kit
CAT# AFG-IVE-14051
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NPY2R ELISA Kit
CAT# AFG-IVE-14050
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NPR2 ELISA Kit
CAT# AFG-IVE-14043
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NPR1 ELISA Kit
CAT# AFG-IVE-14042
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NPPC ELISA Kit
CAT# AFG-IVE-14041
Size: 96 Wells
1,639.00 1639.0 USD
AffiELISA®​ Rat NPPB ELISA Kit
CAT# AFG-IVE-14040
Size: 96 Wells
1,639.00 1639.0 USD
AffiELISA®​ Rat NOG ELISA Kit
CAT# AFG-IVE-14023
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NKAP ELISA Kit
CAT# AFG-IVE-14016
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NGF Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-14008
Size: 96 Wells
1,601.88 1601.88 USD
1,601.88 1601.88 USD
AffiELISA®​ Rat NFE2L2 ELISA Kit
CAT# AFG-IVE-13999
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat NCDN ELISA Kit
CAT# AFG-IVE-13986
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat MYH7 ELISA Kit
CAT# AFG-IVE-13966
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat MX1 ELISA Kit
CAT# AFG-IVE-13958
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat MTND1 ELISA Kit
CAT# AFG-IVE-13945
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat MSTN Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13934
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat MPO Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13921
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat MKI67 ELISA Kit
CAT# AFG-IVE-13893
Size: 96 Wells
1,564.75 1564.75 USD
1,601.88 1601.88 USD
AffiELISA®​ Rat MFAP5 ELISA Kit
CAT# AFG-IVE-13878
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat METRNL ELISA Kit
CAT# AFG-IVE-13876
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat MELK ELISA Kit
CAT# AFG-IVE-13869
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat MC2R ELISA Kit
CAT# AFG-IVE-13856
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat MB Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13850
Size: 96 Wells
1,676.13 1676.13 USD
AffiELISA®​ Rat MAT1A ELISA Kit
CAT# AFG-IVE-13847
Size: 96 Wells
1,564.75 1564.75 USD
1,601.88 1601.88 USD
AffiELISA®​ Rat LTB Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13805
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat LRP8 ELISA Kit
CAT# AFG-IVE-13800
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat LOX ELISA Kit
CAT# AFG-IVE-13788
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat LEP Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13760
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat LEFTY1 ELISA Kit
CAT# AFG-IVE-13759
Size: 96 Wells
1,564.75 1564.75 USD
1,601.88 1601.88 USD
AffiELISA®​ Rat LDHC ELISA Kit
CAT# AFG-IVE-13754
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat KMO ELISA Kit
CAT# AFG-IVE-13724
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat KLF2 ELISA Kit
CAT# AFG-IVE-13714
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat KL ELISA Kit
CAT# AFG-IVE-13712
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat KIF5B ELISA Kit
CAT# AFG-IVE-13705
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat KCNJ5 ELISA Kit
CAT# AFG-IVE-13695
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat JAK3 ELISA Kit
CAT# AFG-IVE-13685
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat ITIH4 ELISA Kit
CAT# AFG-IVE-13676
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat INHBB ELISA Kit
CAT# AFG-IVE-13653
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat INHA ELISA Kit
CAT# AFG-IVE-13651
Size: 96 Wells
1,639.00 1639.0 USD
AffiELISA®​ Rat IL4 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13630
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat IL34 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13628
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat IL33 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13626
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat IL25 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13619
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat IL24 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13617
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat IL21 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13608
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat IL19 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13592
Size: 96 Wells
1,601.88 1601.88 USD
1,601.88 1601.88 USD
AffiELISA®​ Rat IL11RA1 ELISA Kit
CAT# AFG-IVE-13574
Size: 96 Wells
1,564.75 1564.75 USD
1,601.88 1601.88 USD
AffiELISA®​ Rat HSPA1L ELISA Kit
CAT# AFG-IVE-13507
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat HRH4 ELISA Kit
CAT# AFG-IVE-13496
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat HRH1 ELISA Kit
CAT# AFG-IVE-13494
Size: 96 Wells
1,564.75 1564.75 USD
1,601.88 1601.88 USD
1,676.13 1676.13 USD
AffiELISA®​ Rat HGF Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13459
Size: 96 Wells
1,601.88 1601.88 USD
1,601.88 1601.88 USD
1,601.88 1601.88 USD
AffiELISA®​ Rat HAMP High Sensitivity ELISA Kit
CAT# AFG-IVE-13433
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat HAMP ELISA Kit
CAT# AFG-IVE-13432
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat GSAP ELISA Kit
CAT# AFG-IVE-13406
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat GRN ELISA Kit
CAT# AFG-IVE-13402
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat GRN Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13401
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat GRIN1 ELISA Kit
CAT# AFG-IVE-13397
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat GPT Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13387
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat GPAM ELISA Kit
CAT# AFG-IVE-13376
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat GLUL ELISA Kit
CAT# AFG-IVE-13363
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat GLP1R ELISA Kit
CAT# AFG-IVE-13355
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat GLDC ELISA Kit
CAT# AFG-IVE-13352
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat GKN2 ELISA Kit
CAT# AFG-IVE-13350
Size: 96 Wells
1,564.75 1564.75 USD
AffiELISA®​ Rat GKN2 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13349
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat GH1 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13336
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat GDNF Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13328
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat GDF5 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13324
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat GDF3 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13322
Size: 96 Wells
1,601.88 1601.88 USD
AffiELISA®​ Rat GDF2 Chemi-Luminescent ELISA Kit
CAT# AFG-IVE-13320
Size: 96 Wells
1,601.88 1601.88 USD
1,601.88 1601.88 USD
AffiELISA®​ Rat GDF11 ELISA Kit
CAT# AFG-IVE-13317
Size: 96 Wells
1,564.75 1564.75 USD
1,601.88 1601.88 USD