FGF ELISA Kits

Fibroblast Growth Factor (FGF) ELISA kits are specialized immunoassays used for the quantitative detection and measurement of FGF family proteins in biological samples such as serum, plasma, cell culture supernatants, and tissue lysates. FGFs are a group of growth factors involved in a wide range of biological processes, including cell growth, development, angiogenesis, and wound healing. They play critical roles in both normal physiology and pathophysiological conditions such as cancer, fibrosis, and metabolic disorders.

Content of FGF ELISA Kit

• Pre-coated Microplate: A 96-well plate pre-coated with a high-affinity capture antibody specific to FGF.
• Biotinylated Detection Antibody: Ensures precise detection and binding to the FGF protein in samples.
• Enzyme Conjugate: Streptavidin-HRP (horseradish peroxidase) for signal amplification and enhanced detection sensitivity.
• Standards: Calibrated FGF standard solutions for constructing a standard curve for accurate quantification.
• Substrate and Stop Solutions: TMB (3,3’,5,5’-tetramethylbenzidine) as a substrate for color development, and an acidic stop solution to halt the reaction.

Applications of FGF ELISA Kit

• Cancer Research: FGFs, particularly FGF2 (basic FGF), are implicated in tumor growth, angiogenesis, and metastasis. Quantifying FGFs in tumor samples or serum helps in understanding cancer progression and evaluating targeted therapies.
• Wound Healing and Tissue Regeneration Studies: FGFs are critical in tissue repair and regeneration. FGF ELISA kits are used to monitor FGF levels in research focused on regenerative medicine and wound healing.
• Developmental Biology: FGFs are involved in embryonic development and organogenesis. Studying FGF concentrations helps in elucidating developmental pathways and potential congenital disorders.
• Metabolic Disease Research: FGFs, such as FGF21, play a significant role in metabolic regulation and are studied in the context of obesity, diabetes, and metabolic syndrome.
• Drug Development: Evaluating the efficacy of drugs targeting FGF receptors or signaling pathways requires precise measurement of FGF levels in preclinical and clinical samples.

FGF ELISA kits offer high specificity, sensitivity, and reproducibility, making them indispensable tools for advancing research in cell biology, cancer, developmental biology, and metabolic studies.