HSP ELISA Kits

The HSP (Heat Shock Protein) ELISA Kit is a specialized analytical tool designed for the quantitative detection and measurement of specific heat shock proteins in various biological samples. Heat shock proteins play essential roles as molecular chaperones, assisting in protein folding, transport, and preventing protein aggregation under stress conditions. These proteins are integral to the cellular stress response and are upregulated in response to environmental stresses like heat, oxidative damage, and toxic compounds. 

Content of HSP ELISA Kit

• Coated Microplate: The microplate is pre-coated with specific antibodies targeting HSPs, optimizing antigen capture.
• Detection Antibody: This conjugated antibody binds specifically to HSPs, forming a sandwich complex for highly sensitive detection.
• Calibrators and Standards: Provided for the establishment of a standard curve to enable accurate quantification of HSP levels in unknown samples.
• Substrate Solution: TMB (3,3′,5,5′-Tetramethylbenzidine) substrate provides a chromogenic reaction when the enzyme conjugate binds, yielding a measurable color change.

Application of HSP ELISA Kit

The HSP ELISA Kit is utilized in various fields, including:

• Toxicology: Useful for assessing cellular responses to toxic agents, helping to identify compounds that induce stress pathways and could potentially cause cellular damage.
• Oncology: HSPs are linked to tumorigenesis and cancer progression. This ELISA kit aids in profiling HSP expression in cancer cells and tissues, contributing to research on tumor biology, metastasis, and potential therapeutic targets.
• Neuroscience: HSPs are significant in neurodegenerative conditions, where protein aggregation and cellular stress are critical. The kit is used to study HSP regulation in Alzheimer's, Parkinson's, and Huntington's disease models.

The HSP ELISA Kit’s technical design offers rapid, reliable quantification of HSPs, making it a valuable tool for researchers across diverse biomedical fields.