IGG ELISA Kits

IgG ELISA kits are immunoassays designed for the quantitative or qualitative measurement of Immunoglobulin G (IgG) in biological samples such as serum, plasma, and other bodily fluids. IgG is the most abundant type of antibody found in the circulation and plays a crucial role in the immune response by neutralizing pathogens, opsonization, and activating complement. IgG measurements are essential for assessing immune system status, diagnosing various conditions, and monitoring immune responses, such as in vaccine efficacy studies.

Content of IgG ELISA Kit: IgG ELISA kits typically include:

• A 96-well microplate pre-coated with anti-IgG capture antibodies.
• Recombinant IgG standards for generating a standard curve.
• Detection antibodies conjugated with an enzyme such as HRP (Horseradish Peroxidase) for signal amplification.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for developing the colorimetric signal.
• Stop solution to halt the enzymatic reaction.
• Sample diluents, wash buffers, and assay buffers.
• A comprehensive protocol with detailed instructions for sample preparation and assay execution.

Application of IgG ELISA Kit: IgG ELISA kits are used in various research and clinical applications, including:

• Immune status assessment: Measuring total IgG levels to evaluate overall immune health or detect immunodeficiencies.
• Vaccine response studies: Assessing the levels of specific IgG antibodies post-vaccination to determine the immune response and vaccine efficacy.
• Infectious disease diagnosis: Detecting specific IgG antibodies to identify past exposure to pathogens, such as in serological testing for viral, bacterial, and parasitic infections.
• Autoimmune disease research: Investigating elevated or abnormal IgG levels associated with autoimmune disorders.
• Allergy and hypersensitivity studies: Measuring IgG subclasses to understand immune responses related to allergens.
• Monitoring chronic diseases: Evaluating IgG levels in conditions such as multiple myeloma and chronic inflammatory diseases.
• Therapeutic monitoring: Tracking IgG-based treatments, such as intravenous immunoglobulin (IVIG) therapy.

IgG ELISA kits are fundamental tools for researchers and clinicians aiming to assess immune function, diagnose diseases, and evaluate responses to vaccines and therapies, providing precise and reproducible quantitative data.