The KLK (Kallikrein) ELISA Kit is designed for the quantitative measurement of kallikreins in biological samples. Kallikreins are a family of serine proteases that play crucial roles in various physiological and pathological processes, including blood pressure regulation, cancer progression, and tissue remodeling. The KLK ELISA Kit typically targets specific kallikrein isoforms, such as KLK2, KLK3 (PSA), or KLK10, depending on the application.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells pre-coated with an antibody specific to the target kallikrein, allowing for capture of kallikreins from the samples.
- Standards:
- Solutions containing known concentrations of recombinant or purified kallikrein, used to generate a standard curve for quantifying kallikrein levels in samples.
- Sample Diluent:
- A buffer solution used to dilute biological samples to appropriate concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to a different epitope of the kallikrein, often conjugated to an enzyme such as HRP for detection.
- Enzyme Conjugate:
- If the detection antibody is biotinylated, a streptavidin-HRP conjugate may be included for signal detection.
Procedure
- Coating:
- Coat the wells of the microplate with an antibody specific to the target kallikrein. Incubate to allow binding and then wash away unbound antibody.
- Blocking:
- Block any remaining uncoated surfaces in the wells with a blocking buffer to prevent non-specific binding.
- Addition of Standards and Samples:
- Add standards and samples to the wells. The kallikrein in the samples will bind to the immobilized antibody.
- Incubation:
- Incubate the plate to allow kallikrein to bind to the antibody.
- Addition of Detection Antibody:
- Add a detection antibody specific to the kallikrein, which binds to the captured kallikrein.
Performance Characteristics
- Sensitivity: Capable of detecting low levels of kallikreins, typically in the picogram per milliliter (pg/mL) range.
- Precision: Shows low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
- Linearity: Demonstrates linearity across the standard curve range, ensuring accurate quantification.
- Recovery: High recovery rates, usually above 85%, indicating reliable performance across different sample types.
Applications
- Prostate Cancer: Measurement of KLK3 (PSA) levels for prostate cancer diagnosis and monitoring.
- Cardiovascular Diseases: Assessing kallikreins related to cardiovascular health.
- Cancer Research: Studying the role of kallikreins in various types of cancer.
- Tissue Remodeling: Evaluating kallikreins involved in tissue repair and remodeling processes.
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