KLK ELISA Kits

The KLK ELISA Kit is a highly specific immunoassay designed for the quantitative measurement of kallikreins (KLKs), a family of serine proteases, in biological samples. KLKs play significant roles in diverse physiological processes, including skin desquamation, inflammation, and hormone processing, and are particularly known for their involvement in cancer progression, especially prostate, breast, and ovarian cancers. 

Content of KLK ELISA Kit

A standard KLK ELISA Kit includes:

• Standards and Controls: Calibrated standards to create a reliable standard curve, along with positive and negative controls for validating assay accuracy.
• Substrate Solution: Chromogenic or chemiluminescent substrate that reacts with the enzyme to produce a signal proportional to KLK concentration.
• Stop Solution: Stops the enzymatic reaction, ensuring precise and consistent endpoint readings.
• Wash Buffer and Sample Diluent: Solutions to optimize assay conditions, reducing nonspecific binding and background noise.

These components are optimized to provide reliable, reproducible measurement of KLKs across various sample types, including serum, plasma, tissue homogenates, and cell culture supernatants.

Application of KLK ELISA Kit

KLK ELISA Kits have extensive applications in cancer research, diagnostic studies, and other fields related to inflammation and protease activity:

• Inflammation and Immune Response Studies: KLKs are involved in inflammatory processes and immune responses. Quantifying KLK levels helps explore their role in conditions such as inflammatory skin diseases, arthritis, and autoimmune disorders.
• Cardiovascular Disease Research: Certain kallikreins, such as KLK1, play roles in cardiovascular health by influencing blood pressure regulation and vascular function. KLK ELISA Kits are useful for studies focused on hypertension, atherosclerosis, and other cardiovascular diseases.
• Drug Development and Therapeutic Targeting: KLKs are emerging targets for therapeutic intervention in cancer and other diseases. KLK ELISA kits can be used to screen potential drug candidates that inhibit or modulate kallikrein activity, supporting preclinical and clinical research.

The KLK ELISA Kit provides a powerful tool for researchers in diverse fields, offering reliable and precise quantification of kallikreins to advance understanding of their biological roles and therapeutic potential.