The MDC (Macrophage-Derived Chemokine) ELISA Kit is designed for the quantitative measurement of MDC (also known as CCL22) levels in biological samples such as serum, plasma, cell culture supernatants, and tissue homogenates. MDC is a chemokine involved in the regulation of immune responses, particularly in attracting T cells and influencing the behavior of various immune cells. It is often studied in the context of immune diseases, cancer, and inflammatory responses.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells pre-coated with an antibody specific to MDC, allowing capture of MDC from the samples.
- Standards:
- Solutions containing known concentrations of recombinant or purified MDC, used to generate a standard curve for quantifying MDC levels in samples.
- Sample Diluent:
- A buffer solution used to dilute biological samples to appropriate concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to a different epitope of MDC, often conjugated to an enzyme such as HRP for detection.
- Enzyme Conjugate:
- If the detection antibody is biotinylated, a streptavidin-HRP conjugate may be included for signal detection.
- Substrate Solution (TMB):
- Tetramethylbenzidine, which reacts with HRP to produce a colorimetric signal proportional to the amount of MDC.
Assay Procedure Summary
- Sample and Standard Addition: Add standards and samples to the pre-coated wells where MDC is captured by the immobilized antibody.
- Wash: Remove unbound substances by washing the wells.
- Detection Antibody Addition: Add a detection antibody specific to MDC, which binds to the captured protein.
- Wash: Wash away excess detection antibody.
- Enzyme Conjugate Addition: If using a biotinylated detection antibody, add a streptavidin-HRP conjugate to bind to the biotinylated antibody.
- Wash: Remove unbound enzyme conjugate through washing.
Performance Characteristics
- Sensitivity: Capable of detecting low levels of MDC, typically in the picogram per milliliter (pg/mL) range.
- Precision: Shows low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
- Linearity: Demonstrates linearity across the standard curve range, ensuring accurate quantification.
- Recovery: High recovery rates, usually above 85%, indicating reliable performance across different sample types.
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