The MMP (Matrix Metalloproteinase) ELISA Kit is designed to quantitatively measure the levels of matrix metalloproteinases (MMPs) in biological samples such as serum, plasma, tissue homogenates, and cell culture supernatants. MMPs are a family of enzymes involved in the breakdown of extracellular matrix components and play crucial roles in various physiological and pathological processes, including tissue remodeling, wound healing, and cancer metastasis.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells pre-coated with an antibody specific to the target MMP, allowing for capture of MMP from the samples.
- Standards:
- Solutions containing known concentrations of recombinant or purified MMP, used to generate a standard curve for quantifying MMP levels in samples.
- Sample Diluent:
- A buffer solution used to dilute biological samples to appropriate concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to a different epitope of the target MMP, often conjugated to an enzyme such as HRP for detection.
Procedure
- Coating:
- Coat the wells of the microplate with an antibody specific to the target MMP. Incubate to allow binding and then wash away unbound antibody.
- Blocking:
- Block any remaining uncoated surfaces in the wells with a blocking buffer to prevent non-specific binding.
- Addition of Standards and Samples:
- Add standards and samples to the wells. The MMP in the samples will bind to the immobilized antibody.
- Incubation:
- Incubate the plate to allow MMP to bind to the antibody.
- Addition of Detection Antibody:
- Add a detection antibody specific to the MMP, which binds to the captured MMP.
Performance Characteristics
- Sensitivity: Capable of detecting low levels of MMPs, typically in the picogram per milliliter (pg/mL) range.
- Precision: Shows low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
- Linearity: Demonstrates linearity across the standard curve range, ensuring accurate quantification.
- Recovery: High recovery rates, usually above 85%, indicating reliable performance across different sample types.
Applications
- Tissue Remodeling: Study of matrix degradation and remodeling processes in various diseases and physiological conditions.
- Cancer Research: Monitoring MMP levels to understand tumor progression and metastasis.
- Inflammatory Diseases: Assessing MMP activity in diseases involving chronic inflammation.
- Bone Diseases: Investigating bone resorption and other bone-related conditions.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|