Overview of PSMB ELISA Kits
PSMB ELISA Kits are designed for the quantitative measurement of Proteasome Subunit Beta (PSMB) levels in various biological samples. PSMB proteins are critical components of the proteasome complex involved in protein degradation and regulation of various cellular processes.
Technical Content
Key Features:
- High Sensitivity: Capable of detecting low levels of PSMB proteins, ensuring precise quantification even in samples with low expression.
- Specificity: High specificity for PSMB proteins, minimizing cross-reactivity with other proteins.
- Wide Range of Applications: Suitable for use with serum, plasma, cell lysates, and other biological fluids.
Applications:
- Cell Biology Research: Used to study the role of PSMB proteins in proteasome function, protein degradation, and cellular regulation.
- Cancer Research: Facilitates the investigation of PSMB proteins' roles in tumor development, progression, and response to therapy.
- Neurodegenerative Disease Research: Assists in understanding the involvement of PSMB proteins in neurodegenerative conditions like Alzheimer's and Parkinson's diseases.
Usage Protocol:
- Sample Preparation: Collect and prepare samples according to the kit instructions. Dilute if necessary.
- Assay Procedure: Add samples, standards, and controls to the wells of the ELISA plate pre-coated with antibodies specific to the target PSMB protein.
- Incubation: Incubate the plate to allow binding of the PSMB proteins to the antibodies.
- Detection: Add detection antibodies and enzyme substrate to produce a colorimetric signal proportional to the PSMB protein concentration.
- Analysis: Measure the absorbance using a microplate reader and calculate PSMB protein concentrations based on the standard curve.
Benefits:
- Precision: Provides reliable and reproducible results for the accurate quantification of PSMB proteins.
- Efficiency: Simplified protocol with ready-to-use reagents reduces assay time and labor.
- Versatility: Compatible with a variety of sample types, making it suitable for diverse research applications.
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