CCL ELISA Kits

CCL (Chemokine (C-C motif) ligand) ELISA kits are designed for the quantitative measurement of CCL family proteins in various biological samples, such as serum, plasma, cell culture supernatants, and tissue lysates. CCL proteins are chemokines involved in immune cell signaling, recruitment, and inflammation. They play a significant role in immune responses, inflammation, and various disease states, including autoimmune disorders, cancer, and infectious diseases.

Content: CCL ELISA kits typically use the sandwich ELISA format, ensuring high specificity and sensitivity for detecting CCL proteins. Common kit components include:

• 96-well microplate pre-coated with capture antibodies specific for the target CCL chemokine (e.g., CCL2/MCP-1, CCL5/RANTES).
• Standards and controls for creating a standard curve for precise quantification.
• Biotinylated detection antibody, binding to a different epitope of the CCL target.
• Streptavidin-HRP enzyme conjugate for signal amplification.
• TMB substrate solution, producing a color change when catalyzed by HRP.
• Stop solution to terminate the colorimetric reaction.
• Wash buffers and sample diluents for consistency and reproducibility in the assay.

Applications: CCL ELISA kits are widely used in various research and clinical studies, such as:

• Immunology Research: Analyzing the role of CCL chemokines in immune cell recruitment and signaling during immune responses and inflammation.
• Inflammatory Disease Studies: Measuring CCL levels to understand their involvement in conditions such as rheumatoid arthritis, asthma, and inflammatory bowel disease (IBD).
• Cancer Research: Investigating the role of CCL proteins in tumor growth, angiogenesis, and metastasis, as chemokines can influence the tumor microenvironment and immune cell infiltration.
• Infectious Disease Research: Monitoring CCL levels in response to bacterial or viral infections to understand host-pathogen interactions and immune defense mechanisms.

CCL ELISA kits are valuable tools for the accurate quantification of CCL chemokines in various biological samples. Their use in immunology, inflammation, cancer, and autoimmune disease research helps to elucidate the complex roles of chemokines in immune cell signaling, disease progression, and potential therapeutic strategies.